Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 68, Issue 11, Pages 2332-2340Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.68.2332
Keywords
synthesized alpha-glucan; macrophages; anti-tumor activity; tumor necrosis factor-alpha (TNF-alpha)
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Oral administration of an enzymatically synthesized alpha-1,4:1,6-glycogen (ESG) at a dose of 50/mug/ml significantly prolonged the survival time of Meth A tumor-bearing mice. ESG also significantly stimulated macrophage-like cells (J774.1), leading to augmented production of nitric oxide (NO) and tumor necrosis factor-a JNF-alpha). The weight-average degree of polymerization (DPw) and the ratio of branch linkage (BL) of ESG were 149,000 and 8.1% respectively. beta-Amylase-treated ESG, however, lost J774. 1 -activating activity although inhibited subcutaneous growth of Meth A tumor cells admixed with it. Its DPw and BL changed to 126,000 and 20% respectively. Partially degraded amylopectin [(AP), DPw: 110,000, BL; 5.11 was also effective at stimulating J774.1, but its activity was lower than that of ESG. Other alpha-glucans [cycloamylose (CA), enzymatically synthesized amylose (ESA), highly branched cyclic dextrin (HBCD), and beta-amylase-treated HBCD], of which DPw was lower than that of ESG, showed no J774.1 -activating activity and weaker anti-tumor activity.
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