Journal
ULTRAMICROSCOPY
Volume 101, Issue 2-4, Pages 129-138Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultramic.2004.04.004
Keywords
single particles; electron microscopy; image normalization; image formation model; computed tomography
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Funding
- NHLBI NIH HHS [HL740472, HL67465-01] Funding Source: Medline
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In the process of three-dimensional reconstruction of single particle biological macromolecules several hundreds, or thousands, of projection images are taken from tens or hundreds of independently digitized micrographs. These different micrographs show differences in the background grey level and particle contrast and, therefore, have to be normalized by scaling their pixel values before entering the reconstruction process. In this work several normalization procedures are studied using a statistical comparison framework. We finally show that the use of the different normalization methods affects the reconstruction quality, providing guidance on the choice of normalization procedures. (C) 2004 Elsevier B.V. All rights reserved.
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