Journal
JOURNAL OF NEUROBIOLOGY
Volume 61, Issue 2, Pages 222-235Publisher
WILEY
DOI: 10.1002/neu.20038
Keywords
GAP-43; HuD; growth cone; mRNA; ELAV/Hu family; immunocytochemistry; in situ hybridization
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Funding
- NIGMS NIH HHS [R01 GM55599] Funding Source: Medline
- NINDS NIH HHS [R01 NS30255] Funding Source: Medline
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The neuron-specific ELAV/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons, and leads to increased levels of GAP-43 protein. As GAP-43 protein is enriched in growth cones, it is of interest to determine if HuD and GAP-43 mRNA are associated in developing growth cones. HuD granules in growth cones are found in the central domain that is rich in microtubules and ribosomes, in the peripheral domain with its actin network, and in filopodia. This distribution of HuD granules in growth cones is dependent on actin filaments but not on microtubules. GAP-43 mRNA is localized in granules found in both the central and peripheral domains, but not in filopodia. Ribosomes were extensively colocalized with HuD and GAP-43 mRNA granules in the central domain, consistent with a role in the control of GAP-43 mRNA stability in the growth cone. Together, these results demonstrate that many of the components necessary for GAP-43 mRNA translation/stabilization are present within growth cones. (C) 2004 Wiley Periodicals, Inc.
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