Journal
APPLIED PHYSICS LETTERS
Volume 97, Issue 16, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3499652
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Funding
- National Institute of General Medical Sciences [R01GM085437]
- National Science Foundation
- 3Com Corporation
- Bert and DeeDee McMurtry Stanford Graduate Fellowship
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Wide-field microscopy with a double-helix point spread function (DH-PSF) provides three-dimensional (3D) position information beyond the optical diffraction limit. We compare the theoretical localization precision for an unbiased estimator of the DH-PSF to that for 3D localization by astigmatic and biplane imaging using Fisher information analysis including pixelation and varying levels of background. The DH-PSF results in almost constant localization precision in all three dimensions for a 2 mu m thick depth of field while astigmatism and biplane improve the axial localization precision over smaller axial ranges. For high signal-to-background ratio, the DH-PSF on average achieves better localization precision. (C) 2010 American Institute of Physics. [doi:10.1063/1.3499652]
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