Journal
ELECTROPHORESIS
Volume 25, Issue 21-22, Pages 3669-3676Publisher
WILEY
DOI: 10.1002/elps.200406063
Keywords
desorption/ionization on silicon; enzyme inhibitor; immobilized enzyme; matrix assisted laser desorption/ioniozation-time of flight-mass spectrometry; miniaturization; peptide mapping
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Desorption/ionization on silicon mass spectrometry (DIOS-MS) is a matrix-free technique that allows for the direct desorption/ionization of low-molecular-weight compounds with little or no fragmentation of analytes. This technique has a relatively high tolerance for contaminants commonly found in biological samples. DIOS-MS has been applied to determine the activity of immobilized enzymes on the porous silicon surface. Enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. It is demonstrated that this method can be applied to the screening of enzyme inhibitors. Furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix-assisted laser desorption/ionization-time of flight-MS has been developed.
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