Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 60, Issue 13, Pages 3007-3012Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2004.02.018
Keywords
ampicillin sodium; sodium 1,2-naphthoquinone-4-sulfonic; spectrophotometry
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Spectrophotometric determination of ampicillin sodium is described. The ampicillin sodium reacts with sodium 1,2-naphthoquinone-4-sulfonic in pH 9.00 buffer solution to form a salmon pink compound, and its maximum absorption wavelength is at 463 nm, epsilon(463) = 1.14 x 10(4). The absorbance of ampicillin sodium from 2.0-80 mug ml(-1) obeys Beer's law. The linear regression equation of the calibration graph is C = 40.24A - 2.603, with a linear regression correlation coefficient is 0.9997, the detection limit is 1.5 mug ml(-1), recovery is from 97.23 to 104.5%. Effects of pH, surfactant, organic solvents, and foreign ions on the determination of ampicillin sodium have been examined. This method is rapid and simple, and can be used for the determination of ampicillin sodium in the injection solution of ampicillin sodium. The results obtained by this method agreed with those by the official method (HPLC). (C) 2004 Elsevier B.V. All rights reserved.
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