Electron transfer mechanism of cytochrome c at graphene electrode

We report the direct electron transfer of cytochrome c (Cyt c) observed at graphene electrodes. Graphene nanosheets were chemically synthesized and immobilized on to a glassy carbon electrode. Cyclic voltammetry of Cyt c in phosphate buffered saline was performed at these electrodes. Results indicated a pair of reversible redox waves with a peak-to-peak separation value of 0.07 V in a diffusion controlled electrochemical process. Furthermore, the voltammetric response of these electrodes in Cyt c were found to be stable over time with negligible electrode fouling toward Cyt c. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3458698]