Journal
APPLIED PHYSICS LETTERS
Volume 97, Issue 6, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3476356
Keywords
biological techniques; bioMEMS; biotechnology; diseases; DNA; electrophoresis; gels; gene therapy; genetics; genomics; microfluidics; molecular biophysics; patient diagnosis; polymer blends
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Funding
- National Science Council of the Republic of China [NSC 98-2221-E-011-006-MY3]
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In this study, we grafted thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) onto a Si substrate as the medium in a microfluidic device to detect specific DNA molecules [human genomic DNA (hgDNA528), 528 bp] at extremely low concentrations (down to 2 ng/mu l). After using the polymerase chain reaction to amplify the released human gDNA signal from the tethered PNIPAAm on the substrate, the amplified human gDNA molecules were characterized through agarose gel electrophoresis. The tethered PNIPAAm in the fluid device allowed the precise detection of the human gDNA. (C) 2010 American Institute of Physics. [doi:10.1063/1.3476356]
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