Journal
APPLIED PHYSICS LETTERS
Volume 97, Issue 4, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3462326
Keywords
biochemistry; biomedical materials; cellular biophysics; molecular biophysics; plasma materials processing; polymer films; proteins; soft lithography; surface treatment
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Surface patterning for single-cell culture was accomplished by combining plasma-assisted surface chemical modification, soft lithography, and protein-induced surface activation. Hydrophilic patterns were produced on Parylene C films deposited on glass substrates by oxygen plasma treatment through the windows of polydimethylsiloxane shadow masks. After incubation first with Pluronic F108 solution and then serum medium overnight, surface seeding with mesenchymal stem cells in serum medium resulted in single-cell patterning. The present method provides a means of surface patterning with direct implications in single-cell culture. (C) 2010 American Institute of Physics. [doi:10.1063/1.3462326]
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