Journal
SCIENCE
Volume 306, Issue 5699, Pages 1194-1198Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1100709
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Funding
- NCI NIH HHS [P01 CA078048] Funding Source: Medline
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We present a method for high-throughput cytological profiling by microscopy. Our system provides quantitative multidimensional measures of individual cell states over wide ranges of perturbations. We profile dose-dependent phenotypic effects of drugs in human cell culture with a titration-invariant similarity score (TISS). This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism. Multivariate single-cell analysis is a starting point for identifying relationships among drug effects at a systems level and a step toward phenotypic profiting at the single-cell level. Our methods will be useful for discovering the mechanism and predicting the toxicity of new drugs.
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