Journal
APPLIED PHYSICS LETTERS
Volume 96, Issue 4, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3294622
Keywords
biodiffusion; biomolecular effects of radiation; bubbles; cavitation; enzymes; fluorescence; gels; nucleation
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Funding
- Core Research for Evolution Science and Technology (CREST) of JST (Japan Science and Technology Corporation)
- New Energy and Industrial Technology Development Organization (NEDO) of Japan
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Japan Science and Technology Agency
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We found that the use of a gel solution with agarose enhanced femtosecond laser-induced nucleation and produced hen egg white lysozyme crystals at three to five times lower supersaturation than those by the femtosecond laser or agarose alone. The fast fluorescence imaging of the protein in the gel solution revealed that cavitation bubbles created high-concentration regions at the focal point, which could be the trigger for protein nucleation. The lower diffusions of protein molecules in agarose gel retained the high-concentration regions for a longer time, and facilitated the nucleation.
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