Journal
APPLIED PHYSICS LETTERS
Volume 96, Issue 11, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3308485
Keywords
biological techniques; biomolecular effects of radiation; optical microscopy; photothermal effects; proteins; two-photon processes
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Funding
- National Science Foundation [DBI-0649892)]
- U.S Department of Energy's Basic Energy Sciences Program [DE-FG02-07ER15875]
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Heme proteins, such as hemoglobins and cytochromes, play important roles in various biological processes. Here we employ the two-photon excited photothermal effect as a contrast mechanism to map heme proteins distribution. Particularly, both a thermal lens scheme and a high-frequency modulation are utilized to enhance the signal-to-noise ratio. We demonstrate label-free imaging of individual red blood cells, subcellular distribution of cytochromes in live mammalian cells, and the microvascular networks in mouse ear tissue and in a zebrafish gill.
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