Journal
JOURNAL OF NEUROSCIENCE
Volume 24, Issue 46, Pages 10475-10487Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3250-04.2004
Keywords
channel; fluorescence; gating; permeability; purinergic; receptor; P2X; FRET; TIRF
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ATP-gated P2X(2) channels undergo permeability changes through a process that is incompletely understood. In the present study, we used fluorescence resonance energy transfer ( FRET) and electrophysiology to measure cytosolic gating motions in P2X(2) channels as they enter a state with increased permeability. P2X(2) channels underwent permeability changes with a time course that was similar to decreases in FRET between cyan fluorescent protein and yellow fluorescent protein attached to the cytosolic domain of P2X(2) channels. Wild-type and mutant channels that did not undergo permeability changes also did not show evidence of cytosolic gating motions. Moreover, immobilizing the cytosolic domain by tethering it to the plasma membrane prevented the switch in permeability and impaired the cytosolic gating motions. Both of these phenotypes were restored when the immobilizing tether was cleaved. The data provide a time-resolved measure of state-specific gating motions and suggest how a cytosolic domain may control ion channel permeability.
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