Biocompatibility and antibacterial activity of chitosan and collagen immobilized poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid)

Acrylic acid (AA) was grafted to ozone treated poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) membrane. The resulting membranes were further grafted with chitosan (ChS) via esterification. Afterward ChS-grafted membranes were immobilized with collagen (COL). The anti-bacterial activity of chitosan against Staphylococus aureus, Escherichia coli, and Pseudomonas aeruginosa was reserved after COL-immobilization. In addition, after COL-immobilization, the L929 fibroblasts attachment and proliferation to membranes were improved, and the protein adsorption was decreased. The results indicate that by grafting with ChS and immobilizing with COL, PHBV can not only exhibit antibacterial activity, but also improve the cell attachment as well proliferation for fibroblast. (C) 2004 Elsevier Ltd. All rights reserved.