Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1057, Issue 1-2, Pages 95-100Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.09.068
Keywords
isoflavonoid separation; agarose cross-linker; puerarin; acetic acid quenching
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The isoflavonoid puerarin in extracts of the well-known traditional Chinese drug Radix puerariae (root of the plant Pueraria lobata) can be separated from other isoflavonoids by adsorption chromatography on the cross-linked 12% agarose gel Superose 12 equilibrated in distilled water. The adsorption is totally quenched by the addition of 50% acetic acid. The separation of the isoflavonoids is tentatively ascribed to interaction with the residues of the cross-linking reagents used in the manufacturing process of Superose 12. Thus, no useful separation can be achieved with non-cross-linked 12% agarose gel media. Symmetric elution profiles at high sample loadings (16 mg on a 24 ml column) suggest linear adsorption isotherms for the isoflavonoids. (C) 2004 Elsevier B.V. All rights reserved.
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