Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 112, Issue 4, Pages 661-668Publisher
WILEY
DOI: 10.1002/ijc.20461
Keywords
T-lymphocytes; antigenic peptide epitopes; MHC-II; tumor immunity; vaccination
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The SSX2 gene encodes the tumor-specific antigen HOMMEL-40/SSX2 expressed in a broad spectrum of tumors of different origin, against which humoral and CD8(+) T-cell-mediated MHC-1-restricted responses have been demonstrated. Searching for promiscuous MHC-II-restricted peptides that might be suitable as a CD4(+) stimulating vaccine for many patients, we used the SYFPEITHI algorithm and identified a HOM-MEL-40/SSX2-derived pentadecamer epitope (p45-59) that induced specific CD4(+) T-cell responses restricted by the HLA-DRB1 subtypes *0701, *1101 and *1302 that have a cumulative prevalence of similar to25% in the Caucasian population. The CD4(+)-mediated response against p45-59 and its DR restriction was demonstrated by inhibition with anti-CD4 and HLA-DR antibodies, respectively, and by blocking experiments using HLA-specific antibodies. The natural processing and presentation of p45-59 was demonstrated by recognition of the SSX2(+) melanoma cell line Me 275 as well as autologous and allogeneic dendritic cells pulsed with whole-protein SSX2 by T cells with specificity for p45-59. p45-59 was able to induce responses in 3/6 breast cancer patients and 1/5 healthy controls. No correlation was found between CD4(+) T-cell responses against p45-59 reactivity and anti-SSX2 antibody titers in the serum of patients, suggesting that CD4(+) and B-cell responses are regulated independently. p45-59 holds promise as a broadly applicable peptide vaccine for patients with SSX2-positive neoplasms. (C) 2004 Wiley-Liss, Inc.
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