Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1058, Issue 1-2, Pages 113-119Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2004.07.098
Keywords
neuropeptides; leu-enkephalin; quantification
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A standard nanospray-liquid chromatography-tandem mass spectrometry system in a column switching setup for the absolute quantification of leucine-enkephalin was evaluated. Analytes were loaded on a C18 trapping column and back-flushed in the 75 mum analytical column. Quantification was performed with a triple quadrupole instrument. Validation results show that it is feasible, with a conventional nano-LC system in the column switching setup, to quantify peptides as low as 500 amol on column (50 pmol/L). Weighted linear regression analysis proves a good linearity in a dynamic range of almost three orders of magnitude. Nevertheless, robustness remains a key issue in nano-LC-MS/MS. (C) 2004 Elsevier B.V. All rights reserved.
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