Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 24, Issue 23, Pages 10448-10455Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.24.23.10448-10455.2004
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Funding
- NCI NIH HHS [R01 CA096574, R01 CA052461, CA90270, CA96574, CA52461, P30 CA016672, P50 CA090270, CA16672] Funding Source: Medline
- NIEHS NIH HHS [ES07784, P30 ES007784] Funding Source: Medline
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Spindle poisons represent an important class of anticancer drugs that act by interfering with microtubule polymerization and dynamics and thereby induce mitotic checkpoints and apoptosis. Here we show that mammalian SNM1 functions in an early mitotic stress checkpoint that is distinct from the well-characterized spindle checkpoint that regulates the metaphase-to-anaphase transition. Specifically, we found that compared to wild-type cells, Snm1-deficient mouse embryonic fibroblasts exposed to spindle poisons exhibited elevated levels of micronucleus formation, decreased mitotic delay, a failure to arrest in mitosis prior to chromosome condensation, supernumerary centrosomes, and decreased viability. In addition, we show that both Snm1 and 53BP1, previously shown to interact, coimmunoprecipitate with components of the anaphase-promoting complex (APC)/cyclosome. These findings suggest that Snm1 is a component of a mitotic stress checkpoint that negatively targets the APC prior to chromosome condensation.
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