Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 122, Issue 1, Pages 29-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2004.07.008
Keywords
coronavirus; human coronaviruses; RT-PCR assay; SARS
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The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada. (C) 2004 Elsevier B.V. All rights reserved.
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