Journal
EMBO REPORTS
Volume 5, Issue 12, Pages 1171-1175Publisher
WILEY
DOI: 10.1038/sj.embor.7400292
Keywords
ion channel; small G protein; trafficking; recycling endosomes; apical compartment
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TWIK1 belongs to a family of K+ channels involved in neuronal excitability and cell volume regulation. Its tissue distribution suggests a role in epithelial potassium transport. Here we show that TWIK1 is expressed in a subapical compartment in renal proximal tubules and in polarized MDCK cells. In nonpolarized cells, this compartment corresponds to pericentriolar recycling endosomes. We identified EFA6, an exchange factor for the small G protein ADP-ribosylation factor 6 (ARF6), as a protein binding to TWIK1. EFA6 interacts with TWIK1 only when it is bound to ARF6. Because ARF6 modulates endocytosis at the apical surface of epithelial cells, the ARF6/EFA6/TWIK1 association is probably important for channel internalization and recycling.
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