Journal
JOURNAL OF BIOMOLECULAR SCREENING
Volume 9, Issue 8, Pages 695-703Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1087057104267956
Keywords
HDM2; Mdm2; ubiquitin; E3; p53
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An assay for the autoubiquitination activity of the E3 ligase HDM2 (Mdm2) was developed and adapted to a high-throughput formal to identify inhibitors of this activity. The assay can also be used to measure the activity of other E3s and may be useful in finding both inhibitors and activators of a wide range of different ubiquitin ligases.
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