Journal
APPLIED PHYSICS LETTERS
Volume 94, Issue 14, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3111429
Keywords
biochemistry; biosensors; charge exchange; electrochemical electrodes; electrochemical sensors; enzymes; II-VI semiconductors; molecular biophysics; nanobiotechnology; nanofabrication; reaction rate constants; semiconductor thin films; sol-gel processing; voltammetry (chemical analysis); wide band gap semiconductors; zinc compounds
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Nanostructured zinc oxide (nano-ZnO) film has been fabricated onto indium tin oxide (ITO) containing preferred (002) plane and 10 nm crystallite size using sol-gel technique for immobilization of cholesterol oxidase (ChOx). Electrochemical response of ChOx/nano-ZnO/ITO bioelectrode determined as a function of cholesterol concentration using cyclic voltammetry technique reveals improved detection range (5-400 mg/dl), low detection limit (0.5 mg/dl), fast response time (10 s), sensitivity (0.059 mu A/mg dl(-1) cm(-2)), and low value (0.98 mg/dl) of Michaelis-Menten constant (K-m). It is shown that nano-ZnO film provides better environment and enhanced electron transfer between ChOx and electrode.
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