Journal
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
Volume 118, Issue 6, Pages 361-363Publisher
SPRINGER
DOI: 10.1007/s00414-004-0473-0
Keywords
ACTBP2; mutation; primer binding region; multiplex; database
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In the course of routine genotyping of forensic reference samples by multiplex PCR, an allelic drop-out due to mutations in the primer binding regions of the highly polymorphic STR marker ACTBP2 was observed in 17 samples. The variation rate was estimated to be 0.0014 (95% confidence interval: 0.0006 - 0.003). The most frequently found mutation was an G to A transition in the reverse primer binding region which was present in 14 out of 17 cases. To overcome the problem we have added a modified reverse primer to different multiplex kits that led to the correct genotype.
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