Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 54, Issue 6, Pages 1057-1061Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkh470
Keywords
polymyxin resistance; susceptibility testing methodology; interpretative criteria
Ask authors/readers for more resources
Objectives and methods: With their potent activity against Gram-negative bacteria, the polymyxins are important alternative antibiotics for cystic fibrosis (CF) patients. A retrospective evaluation of polymyxin activity against 6001 Pseudomonas aeruginosa, 150 Achromobacter xylosoxidans and 506 Stenotrophomonas maltophilia CF isolates was initiated. In addition, we looked at how polymyxin susceptibility testing was affected by the testing method (agar dilution versus microdilution), the agent (polymyxin E versus polymyxin B), incubation time (24 h versus 48 h) and by different interpretative criteria (German DIN, French FSM, British BSAC). Results: Polymyxin B exhibited reasonable activity against P. aeruginosa (MIC(90)less than or equal to2 mg/L), whereas it was less active against A. xylosoxidans (MIC(90)less than or equal to16 mg/L) and S. maltophilia (MIC(90)less than or equal to16 mg/L). During 2000-2002, polymyxin B resistance in P. aeruginosa, S. maltophilia and A. xylosoxidans was found to be 6.7%, 17.0% and 29.9% (corresponding to 12.4%, 20.7% and 35.4% of infected patients), respectively. When the agar dilution method was used, polymyxin E exhibited higher MICs than polymyxin B. The microdilution method produced lower polymyxin MICs than the agar dilution method. Therefore, the microdilution MICs after prolonged incubation (48 h) and the agar dilution MICs of polymyxin B correlated best (AUC of 0.93, r(2) of 0.44 and s of 0.83). Conclusions: Polymyxin resistance among common CF pathogens is not rare, thus underlining the necessity of accurate susceptibility testing. When compared with the agar dilution method, it was found that the microdilution method is a valid, rapid and cost effective alternative for the determination of polymyxin activity. The performance of the microdilution method was most reliable after prolonged incubation (48 h) at a susceptibility breakpoint of less than or equal to4 mg/L according to the BSAC guidelines (specificity 91%, sensitivity 89%, 1.5% very major errors).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available