Journal
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
Volume 56, Issue 3, Pages 159-171Publisher
ELSEVIER GMBH
DOI: 10.1016/j.etp.2004.08.002
Keywords
glia; lectin binding sites; neurofilament; neocortex; hippocampus; corpus callosum; autolysis; mouse brain
Categories
Ask authors/readers for more resources
The aim of this work was to compare the results of histochemical and immunohistochemical methods using mouse brains which were fixed with various post-mortem delays and storage temperatures (at a constant 4degreesC or 22degreesC, or at gradually decreasing post-mortem temperatures, mimicking conditions of human corpse). We studied the effects of post-mortem delay on glial fibrillary acidic protein, extracellular matrix components to which Wisteria floribunda agglutinin binds, non-phosphorylated neurofilament H, synaptophysin, calbindin and nitric oxide synthase isoenzymes. At the light microscopic level first signs of post-mortem changes were detectable after 6 h. Glial fibrillary acidic protein was most affected by post-mortem delay since its immuno reactivity increased dramatically with increasing post-mortem delay. N-acetylgalactosamines-beta1 labeled lectin binding sites, calbindin and intraneuronal non-phosphorylated neurofilament H seemed to be stable up to 12 h post-mortem. Storage temperature influenced the NADPH-d activity and the content of synaptophysin immuno reactivity to higher degree than all of the other parameters. We found only marginal differences of alterations comparing neocortex, hippocampus and corpus callosum. Our results indicate that different antigens are affected differently by the ongoing catabolic processes during post-mortem delay. (C) 2004 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available