Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 70, Issue 12, Pages 7545-7549Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.12.7545-7549.2004
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We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template rearmealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealing step would cause the bias.
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