Journal
SURFACE SCIENCE
Volume 573, Issue 1, Pages 140-145Publisher
ELSEVIER
DOI: 10.1016/j.susc.2004.05.140
Keywords
alcohols; polycrystalline thin films; biological compounds
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Choline oxidase has been immobilized inside nanostructured polyaniline layers of a controlled porosity and a micrometer or nanometer thickness to form a choline sensor: polyaniline-choline oxidase (PChO) biosensor. Electrochemical techniques were used either, to prepare polyaniline films of a controlled thickness and to immobilize the enzyme molecules. The electrical response of a PChO sensor depends on choline concentration and its sensitivity reaches a value of 5 muA/mM in the amperometric mode and of 10 mV/mM in the potentiometric mode of measurements. The current response measured at a constant potential of +0.4 V is linear vs. choline concentration. No interference with ascorbic acid has been found. The storage stability is very satisfied: the sensors examined did not change essentially their electrical response even after a month of storage. (C) 2004 Elsevier B.V. All rights reserved.
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