Journal
MICROCHIMICA ACTA
Volume 148, Issue 3-4, Pages 227-233Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-004-0265-8
Keywords
enrofloxacin; solid-phase spectrofluorimetry; antibacterials; fluoroquinolones; serum analysis
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A method for the determination of trace amounts of enrofloxacin based on solid-phase spectrofluorimetry has been developed. The relative fluorescence intensity of enrofloxacin fixed on Sephadex SP C-25 gel was measured directly after packing the gel beads in a 1-mm silica cell, using a solid-phase attachment. The wavelengths of excitation and emission were 277 and 448 nm, respectively. The linear concentration range of application was 0.2-4.0 ng mL(-1) of enrofloxacin, with a relative standard deviation of 1.2% (for a level of 2.0 ng mL(-1)) and a detection limit of 0.04 ng mL(-1). The method was applied to the determination of enrofloxacin in commercial pharmaceutical formulations and spiked canine serum samples. It was validated using HPLC as a reference method and applying the standard addition methodology. Recovery levels of the method reached 100% in all cases.
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