Journal
BIOTECHNOLOGY LETTERS
Volume 26, Issue 24, Pages 1861-1865Publisher
SPRINGER
DOI: 10.1007/s10529-004-5320-3
Keywords
cDNA; cloning; D-lactonohydrolase; eukaryotic expression; Fusarium moniliforme
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A D-lactonohydrolase gene of about 1.1 kb was cloned from Fusarium moniliforme. The ORF sequence predicted a protein of 382 amino acids with a molecular mass of about 40 kDa. An expression plasmid carrying the gene under the control of the triose phosphate isomerase gene promotor was introduced into Saccharomyces cerevisiae, and the D-lactonohydrolase gene was successfully expressed in the recombinant strains.
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