Journal
PHYTOCHEMISTRY
Volume 65, Issue 24, Pages 3187-3198Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2004.10.015
Keywords
maize; metabonomics; assay for genetic modification; nuclear magnetic resonance; principal component analysis; partial least squares-discriminant analysis
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The aim of this research was to verify the possibility of identifying and classifying maize seeds obtained from transgenic plants, in different classes according to the modification, on the basis of the concerted variation in metabolite levels detected by NMR spectra. It was possible to recognise the discriminant metabolites of transgenic samples as well as to classify non-a priori defined samples of maize. It is important to underline that the obtained results are useful to point out the metabolic consequences of a specific genic modification on a plant, without using a targeted analysis of the different metabolites, in fact it was possible to classify the seeds also without the complete assignment of the spectra. The analysis was performed by applying multivariate techniques (principal component analysis and partial least squares-discriminant analysis) to NMR data. (C) 2004 Elsevier Ltd. All rights reserved.
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