Journal
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
Volume 24, Issue 12, Pages 2384-2390Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/01.ATV.0000146811.19029.fb
Keywords
atherosclerosis; brachiocephalic artery; apoE -/- mice; MRI; lipid-rich/necrotic core
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Funding
- British Heart Foundation [PS/02/002/14893] Funding Source: Medline
- British Heart Foundation [PS/02/002/14893] Funding Source: researchfish
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Objective - To investigate the ability of high-resolution MRI to determine composition and microanatomy of atherosclerosis in mouse aortic root and brachiocephalic artery. Methods and Results - Aortic root and brachiocephalic arteries of apolipoprotein E knockout (apoE-/-) mice fed Western diet for 10, 20, or 30 weeks were imaged ex vivo (11.7 T; 3D multiecho sequence; resolution 47 x 47 x 62.5 mum). Using semiautomated histogram-based methods, MRI accurately quantified lipid-rich/necrotic areas in the aortic root (r(2) = 0.84; P < 0.001) and brachiocephalic artery (r(2) = 0.90; P < 0.001) compared with histology. Similarly, cell-rich caps in aortic roots, quantified by MRI and histology, correlated closely (r(2) = 0.74; P < 0.001). Reconstruction of segmented brachiocephalic arteries in 3D provided unique insights into plaque microanatomy and enabled volumetric quantification of plaque and lipid-rich/necrotic core. Between 10 and 30 weeks, 3D measurement identified an 11.6-fold increase in plaque volume (versus 4.1-fold for 2D) and a 21.3-fold increase in plaque lipid-rich/necrotic core volume (versus 6.4-fold for 2D), indicating superior power of 3D quantification. Conclusions - Ex-vivo high-resolution 3D MRI accurately quantified lipid-rich/ necrotic core and cell-rich cap areas in atherosclerotic lesions in apoE-/- mice. Reconstruction and volumetric quantification of segmented brachiocephalic arteries demonstrated greater sensitivity in detecting changes in plaque size and lipid composition over time than 2D analysis.
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