Journal
JOURNAL OF BIOMOLECULAR SCREENING
Volume 9, Issue 8, Pages 712-718Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1087057104269495
Keywords
human umbilical vein endothelial cells (HUVEC); vascular endothelial growth factor (VEGF); automated cell migration (ACM)
Ask authors/readers for more resources
By combining the use of BD Biosciences FluoroBlok(TM) membrane-based Boyden chambers with the Cellomics HCS ArrayScan, a more sensitive method for measuring cell migration has been developed. This assay is based on counting nuclei of migrated cells on the bottom of the filter rather than conventional approaches, which use measurement of total well fluorescence. This cell migration assay provides approximately 10-fold increased signal/background compared to conventional approaches and can be used to assess the effects of growth factors on endothelial cell migration and to screen chemical compounds for inhibitory effects on growth factor-mediated endothelial cell migration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
