Journal
BIOPHYSICAL JOURNAL
Volume 87, Issue 6, Pages L3-L5Publisher
CELL PRESS
DOI: 10.1529/biophysj.104.052175
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Funding
- NIDDK NIH HHS [DK60623, F32 DK010181, DK10181, DK47919, R01 DK047919, R01 DK060623] Funding Source: Medline
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We compared secretion kinetics for four different fluorescent cargo proteins, each targeted to the lumen of insulin secretory vesicles. Upon stimulation, individual vesicles displayed one of four distinct patterns of fluorescence change: i), disappearance, ii), dimming, iii), transient brightening, or iv), persistent brightening. For each fusion protein, a different pattern of fluorescence change dominated. Furthermore, we demonstrated that the dominant pattern depends upon both i), the specific choice of fluorescent protein, and ii), the sequence of amino acids linking the cargo protein to the fluorescent protein. Thus, in beta-cells, experiments involving fluorescent cargo proteins for the study of exocytosis must be interpreted carefully, as design of a fluorescent cargo protein determines secretion kinetics at exocytosis.
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