Journal
FEMS MICROBIOLOGY LETTERS
Volume 241, Issue 1, Pages 57-65Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2004.10.004
Keywords
Helicobacter pylori; lipopolysaccharide; Lewis antigen; glycosyltransferase; core oligosaccharide
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Funding
- NIAID NIH HHS [R01 AI41548, R01 AI51291] Funding Source: Medline
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The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an alpha(1,2/3)-glucosyltransferase whereas HP1416 encodes an alpha(1,2/4)-glucosyltransferase. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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