Positive end-expiratory pressure above lower inflection point minimizes influx of activated neutrophils into lung

Objectives. To compare the effects of low vs. high tidal volume (VT) with three positive end-expiratory pressure (PEEP) strategies on activated neutrophil influx into the lung. Design. Prospective, randomized controlled animal study. Setting. Animal laboratory in a university hospital. Subjects. Newborn piglets. Interventions. Surfactant-depleted piglets were randomized in littermate pairs; to PEEP of either 0 (zero end-expiratory pressure [ZEEP]; n = 6), 8 cm H2O (PEEP 8; n = 5), or 1 cm H2O above the lower inflection point (LIP) (PEEP>LIP; n = 6). Within each pair piglets were randomized to a low VT (5-7 mL/kg) or high VT strategy (17-19 mL/kg). After 4 hrs of mechanical ventilation, 18-fluorodeoxyglucose ((18)FDG) was injected and positron emission tomography scanning was performed. Measurements and Main Results. VT and PEEP changes on influx constants of (18)FDG were assessed by analysis of variance. A within-litter comparison of VT was nonsignificant (p = .50). A between-litter comparison, ordered in linear trend rank, from ZEEP, to PEEP 8, to PEEP>LIP, showed a strong effect of PEEP on influx constant (p = .019). Conclusions: PEEP set above the LIP on the inspiratory limb of the pressure-volume curve affords a stronger lung protection than VT strategy.