Journal
MICROSCOPY RESEARCH AND TECHNIQUE
Volume 65, Issue 6, Pages 282-286Publisher
WILEY-LISS
DOI: 10.1002/jemt.20133
Keywords
TOF-SIMS; cholesterol; cryoultramicrotomy of tissue; localization; metal deposition
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The imaging time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) method was utilized to address the problem of cholesterol localization in rat tissues. Rat kidneys were fixed, cryoprotected by sucrose, frozen, sectioned by cryoultramicrotomy, and dried at room temperature. The samples were either covered with a thin silver layer or analyzed uncovered in an imaging TOF-SIMS instrument equipped with an Au-1-3(+)-source. The yield of desorbed secondary ions for some species was up to 600-fold higher after silver coating of the samples. Reference samples of cholesterol were silver-coated and analyzed by TOF-SIMS to define significant peaks, specific for cholesterol. Such peaks were found at m/z = 386 (C27H46O+), m/z = 493 ((C27H46OAg+)-Ag-107), m/z = 495 ((C27H46OAg+)-Ag-109), m/z = 879 ((C54H92O2Ag+)-Ag-107), and m/z = 881 ((C54Hg92O2Ag+)-Ag-109). The silver-cationized cholesterol (493 less than or equal to m/z less than or equal to 495) signal was localized by imaging TOF-SIMS in the kidney sections and showed a high cholesterol content in the kidney glomeruli. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells in the proximal tubules of rat kidney. (C) 2005 Wiley-Liss, Inc.
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