Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 122, Issue 1, Pages 119-122Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2004.08.008
Keywords
influenza A virus; haemagglutinin; subtype; reverse transcription-polymerase chain reaction (RT-PCR)
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Influenza A viruses are subtyped conventionally according to the antigenic characteristics of the external glycoproteins, haemagglutinin (HA) and neuraminidase (NA). To date 15 HA and 9 NA subtypes have been described. There is a need to develop fast, accurate and reliable methods to identify influenza virus subtypes, which may be associated with disease outbreaks. An RT-PCR is described using a single primer pair based on a conserved region of the HA2 gene that can detect all 15 HA influenza A subtypes. The assay was validated initially using a panel of 12 known standard prototype strains of influenza virus representing 6 HA subtypes and subsequently in a blind study using a panel of 30 strains. Selected viruses represented all known HA subtypes derived from avian, swine and human hosts separated both geographically and with time Sequence analysis of RT-PCR product showed complete correlation with results obtained using conventional serological methods. It is concluded that this RT-PCR is a reliable, robust and reproducible tool for the rapid identification of a wide range of all the HA subtypes of influenza A viruses. Crown Copyright (C) 2004 Published by Elsevier B.V. All rights reserved.
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