Journal
IEEE TRANSACTIONS ON NANOBIOSCIENCE
Volume 3, Issue 4, Pages 237-242Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2004.837899
Keywords
biological cells; fluorescence microscopy; three-dimensional (3-D) tracking; trafficking pathway
Funding
- NIAID NIH HHS [R01 AI039167-12, R01 AI050747, R21 AI 53748, R01 AI050747-03, R21 AI053748-02, R01 AI 50747, R21 AI053748, R01 AI039167] Funding Source: Medline
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The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.
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