Quantitative variation in monoterpenes in four species of conifers

Eighteen monoterpenes found in bole and foliage volatiles of four sympatric species of conifers, Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco, lodgepole pine, Pinus contorta var. latifolia Engelm., interior spruce, Picea engelemannii x glauca, and interior fir, Abies lasiocarpa x bifolia, in three locations in British Columbia, Canada, were analysed for quantitative variation (N = 10 trees per sample) using standard and chiral gas chromatography. There was significant variation in monoterpene composition between bole and foliage in all four species. Principal components analysis revealed that the monoterpene profile of coastal Douglas-fir was significantly different from trees in the interior, supporting the hypothesis that coastal and interior populations represent distinct chemotypes. Although the four species did not differ qualitatively, there were significant differences in their quantitative monoterpene profiles in both bole and foliage. These differences, were large enough to suggest that host selection by four species of coniferophagous bark beetles (Coleoptera: Scolytidae), specific to each of the above tree species, may depend on part on perception of, and behavioural response to quantitatively distinct blends of monoterpenes. (C) 2004 Elsevier Ltd. All rights reserved.