Journal
PROTOPLASMA
Volume 224, Issue 3-4, Pages 255-260Publisher
SPRINGER WIEN
DOI: 10.1007/s00709-004-0070-6
Keywords
bafilomycin A; concanamycin A; tobacco BY-2 cells; Golgi apparatus; vacuolation
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Concanamycin A and bafilomycin A are well-known inhibitors of V-ATPase activity. It is known that they interfere with intracellular protein trafficking in both animal and plant cells, but a cellular target for their action in plant cells has not been defined. Here we show that treatment with these inhibitors leads to a massive vacuolation of the Golgi apparatus. The effect is similar, but not identical, to that previously described for the Na+/K+ ionophores and is reversible after washing.
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