Journal
CHEMISTRY & BIOLOGY
Volume 11, Issue 12, Pages 1619-1623Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2004.09.010
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Funding
- NIGMS NIH HHS [GM062594] Funding Source: Medline
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MicroRNAs are small similar to22 nucleotide regulators of numerous biological processes and bind target gene messenger RNAs to control gene expression. The C. elegans microRNA let-7 and its target lin-41 were the first microRNA::target interaction to be validated in vivo. let-7 molecules form imperfect duplexes with two required let-7 complementary sites in the lin-41 3' UTR. Here, we show that base pairing at both the 5' and 3' ends of the let-7 binding site, as well as the presence of unpaired RNA residues in the predicted duplexes, are required for lin-41 downregulation. In this study, our model for microRNA::target interactions also demonstrates that the context of a microRNA binding can be critical for function, revealing an unforeseen complexity in microRNA::target interactions.
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