Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1059, Issue 1-2, Pages 191-198Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2004.09.090
Keywords
capillary zone electrophoresis; ionic liquids; 1-butyl-3-methylimizolium tetrafluoroborate; beta-cyclodextrin; anthraquinones
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The present study reported the ionic liquid (IL) used as running electrolyte in capillary zone electrophoresis (CZE) with beta-cyclodexttin (beta-CD) as modifier for the separation of anthraquinones extract of Chinese herb Paedicalyx attopevensis Pierre ex Pitard. The optimum running electrolyte was 60 mM 1-butyl-3-tnethylimizolium tetrafluoroborate (1B-3MI-TFB) solution with 4.0 mM beta-CD. The pH was 10.00 and the applied voltage was 20 kV with detection at 254 nm. The present method was compared with others and the effect of Joule heating was discussed as well. More significantly, this method is the development of the ionic liquids application to the capillary electrophoresis. (C) 2004 Elsevier B.V. All rights reserved.
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