Journal
EMBO JOURNAL
Volume 23, Issue 24, Pages 4868-4875Publisher
WILEY
DOI: 10.1038/sj.emboj.7600469
Keywords
checkpoint; DNA repair; DNA replication; nonhomologous end-joining; yeast
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DNA double-strand breaks (DSBs) are dangerous lesions that can lead to genomic instability and cell death. Eukaryotic cells repair DSBs either by nonhomologous end-joining (NHEJ) or by homologous recombination. We investigated the ability of yeast cells (Saccharomyces cerevisiae) to repair a single, chromosomal DSB by recombination at different stages of the cell cycle. We show that cells arrested at the G(1) phase of the cell cycle restrict homologous recombination, but are able to repair the DSB by NHEJ. Furthermore, we demonstrate that recombination ability does not require duplicated chromatids or passage through S phase, and is controlled at the resection step by Clb-CDK activity.
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