Journal
JOURNAL OF CATALYSIS
Volume 228, Issue 2, Pages 282-287Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcat.2004.09.010
Keywords
glucose oxidase; kinetics; activation energy; Michaelis-Menten equation
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The selective oxidation of D-glucose to D-gluconic acid was performed at atmospheric pressure, controlled pH value, and constant oxygen concentration, in the temperature range from 273.2 to 303.2 K, using Hyderase (a commercial glucose oxidase and catalase preparation). Measurements of initial rate as a function of initial gluco se concentration were interpreted by a simplified version of the already proposed reaction mechanism, having the form of a simple equation of the Michaelis-Menten type, with two kinetic parameters, i.e., k(1) referring to the reaction between glucose and oxidized enzyme, and k(c) referring to a combination of the other steps. The activation energy for k(1) was found to be 49.6 kJ mol(-1); an apparent activation energy of 26.7 kJ mol(-1) was obtained for kc. (C) 2004 Elsevier Inc. All rights reserved.
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