Journal
BIOSENSORS & BIOELECTRONICS
Volume 20, Issue 6, Pages 1126-1133Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2004.05.017
Keywords
LB films; immunoglobulin G; octadecylamine; behenic acid; ac impedance; immunosensors
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Langmuir-Blodgett (LB) technique appears to be quite suitable for generating biospecific surfaces and it has potential application for fabricating biosensors. In this work, mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile have been transferred onto hydrophobic silver surface previously modified by I-octadecanethiol (ODT) SAMs. In order to obtain stable LB films, the influences of different parameters - type of amphiphile, surface pressure and pH - on the properties of mixed IgG/amphiphile monolayer. were investigated. Electrochemical properties of the engineered immunosensor have been measured by impedimetric spectroscopy. The immunosensor obtained exhibits a high sensitivity and a good specificity in a linear dynamic range from 200 to 1000 ng ml(-1). (C) 2004 Elsevier B.V. All rights reserved.
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