Journal
FEMS MICROBIOLOGY LETTERS
Volume 241, Issue 2, Pages 207-214Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2004.10.022
Keywords
Coniothyrium minitans; REMI; Agrobacterium tumefaciens-mediated transformation; pathogenicity; mycoparasitism
Categories
Ask authors/readers for more resources
Restriction enzyme mediated integration (REMI) and Agrobacterium-mediated transformation (ATMT) were used to transform protoplasts or germinated conidia of the mycoparasite Coniothyrium minitans to hygromycin resistance. Using REMI, up to 32 transformants mug DNA(-1) were obtained, while 37.8 transformants 5 x 10(5) germlings(-1) were obtained using ATMT. Single-copy integrations occurred in 8% and 40% of REMI and ATMT transformants, respectively. A novel microtitre plate-based test was developed to expedite screening of 4000 REMI and ATMT C minitans transformants. Nine pathogenicity mutants that displayed reduced or no pathogenicity on sclerotia of Sclerotinia sclerotiorum were identified. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available