Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 52, Issue 26, Pages 8086-8093Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf049364r
Keywords
apple; Malus domestica; SPME; volatile esters; GC-MS; alpha-farnesene; skin color
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Solid-phase microextraction (SPME) with a polydimethylsiloxane fiber coupled with gas chromatography-mass spectrometry (GC-MS) was applied to the study of variability in volatiles released by 13 apple varieties. The relative amounts of 40 esters and cc-farnesene were determined. Principal component analyses of these results clustered the apples into three groups according to skin color: red, green, and red-green. Total ester contents were highest with the red cluster apples, and the green cluster apples had the highest alpha-farnesene levels. This technology was also applied to the monitoring of changes in volatiles for apples removed from control led-atmosphere storage with subsequent storage at 4 degreesC and room temperature, Total ester contents increased 25-fold, with the greater increases coming at room temperature, whereas a-farnesene levels increased only 5-fold. For apples stored at room temperature, after 11 days, the amount of increase was inversely proportional to the size of the ester: levels of smallest esters (molecular weight 116) increased 12.5-fold, and the largest esters (molecular weight 228) increased similar to1.3-fold.
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