Rapid and transient activation of transcription of the ERF3 gene by wounding in tobacco leaves -: Possible involvement of NtWRKYs and autorepression

This study investigated the regulatory mechanism of rapid and transient induction of a transcriptional repressor ERF3 gene by wounding in tobacco ( Nicotiana tabacum) leaves. Deletion and mutation analysis of the promoter region have suggested that the proximal W boxes (TGAC(C/T)) and a GCC box, respectively, may be involved in the positive and negative regulation of wound-induced expression of the ERF3 gene. Electrophoretic mobility shift assays indicated that wounding enhanced the specific binding activity of nuclear factors to the W boxes. NtWRKY1, -2, and -4, which are tobacco group I WRKYs, interacted specifically with the W boxes and activated transcription via the W boxes. On the other hand, deletion of the GCC box from NsERF3 promoter-GUS reporter gene caused a delay in down-regulation of transcription after wound induction. In addition, ERF3 repressed transcription via the NsERF3 promoter activated by NtWRKYs. These results suggest the possible involvement of NtWRKYs and autorepression in the rapid and transient expression of the ERF3 gene by wounding.