Journal
ADVANCED DRUG DELIVERY REVIEWS
Volume 57, Issue 1, Pages 79-93Publisher
ELSEVIER
DOI: 10.1016/j.addr.2004.05.004
Keywords
imaging; neurotics; organotypic cultures; gene expression; viral vectors; adenovirus; lentivirus
Categories
Ask authors/readers for more resources
Studies of central neurones and other cellular components of the brain, such as glial and vascular cells, can be greatly advanced by the use of the modem optical techniques such as confocal live cell imaging. Fluorescent proteins have allowed imaging of particular cell types or intracellular elements to be visualised and distinguished from irrelevant background structures. To introduce the genetic information encoding for fluorescent proteins into relevant cellular targets, molecular tools are required. Viral vectors are one of the best ways of gene delivery into differentiated postnatal brain neurones and glia. Current progress in this field allows targeting of various cell types and therefore makes it possible to express a variety of fluorescent constructs in selected subpopulations of neurones, for example. In this review, we will discuss and compare the properties of the most popular viral gene delivery systems and the advantages of different brain cell preparations to illustrate how they can be used for high-resolution live cell confocal imaging in order to study new aspects of central nervous system (CNS) structure and function. (C) 2004 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available