Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 201, Issue 1, Pages 127-137Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20041201
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Funding
- NIDDK NIH HHS [DK61331, R21 DK061331] Funding Source: Medline
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The CD4(+)CD25(+) regulatory T cells play a critical role in controlling autoimmunity, but little is known about their development and maintenance. In this study, we investigated whether CD4(+)CD25(-) cells can convert to CD4(+)CD25(-) regulatory T cells in vivo under natural conditions. CD4(+)CD25(-) cells from CD45.1(+) mice were sorted and transferred into congenic CD45.2(+) mice. Converted CD4(+)CD25(+) cells could be detected in lymphoid organs as early as 1 wk after transfer and by 6 wk after transfer, 5-12% of transferred CD4(+) cells expressed CD25. Converted CD4(+)CD25(+) cells themselves failed to proliferate after stimulation, but could suppress proliferation of responder cells in vitro, and also expressed high levels of Foxp3 mRNA. In addition, CD4(+)CD25(-) cells transferred into thymectomized congenic mice converted to CD4(+)CD25(+) cells that also suppressed responder cell proliferation in vitro, and expressed high levels of Foxp3 mRNA. Finally, CD4(+)CD25(-) cells transferred into B7(-/-) mice failed to convert into CD4(+)CD25(+) cells that exhibit the regulatory phenotype. These data indicate that CD4(+)CD25(-) cells convert into CD4(+)CD25(+) regulatory T cells spontaneously in vivo and suggest that this conversion process could contribute significantly to the maintenance of the peripheral CD4(+)CD25(-) regulatory T cell population.
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